玉兰减数分裂观察及染色体构型分析     

李建强  何子灿 《中国科学院研究生院学报》2003,41(4):362-368

采用去壁低渗方法，观察研究了玉兰Magnolia denudata有丝分裂和减数分裂的细胞学特征。实验结果证实玉兰存在两种染色体倍性，即2n=4x=76和2n=6x=114。通常，在木兰属甚至整个木兰科每个物种只具有一种染色体数目。玉兰有丝分裂间期核为复杂染色中心型，其中期染色体较小。玉兰在减数分裂中期I的构型表现出多样性，其中最主要的特点是比同源多倍体预期的二价体出现的频率更高些，其次是在减数分裂中期I可以观察到1或2个环状和（或）链状六价体。这些特征与同源异源六倍体或部分的异源六倍体种北美红杉Sequ  相似文献   

羊皮下脚料中明胶和水解蛋白的提取研究   总被引：1，自引：0，他引：1  

赵宏樵  王方国 《科技通报》1995,11(3):171-174

采用化学及生物化学处理方法，从羊皮下脚料中提取明胶和水解蛋白，通过正交多因子试验，确定最佳化学反应条件及分离方法，试验结果证明：明胶的提取率为８％，水解蛋白的提取率为２５％。  相似文献   

Effect of thiazide drug compound on the testicular protein and cholesterol contents of albino rat     

Hemlata Yadav  P. K. Singh 《Indian journal of clinical biochemistry : IJCB》2003,18(2):206-208

In this study the effect of thiazide diuretic compound on the protein and cholesterol contents in the testes of albino rats as the experimental model. The drug thiazide was administered orally daily for 10,20 and 30 days at the dose of 100mg/kg body weight. Total protein decrease in the testes of rats were evidenced may be due to the side effects of thiazide drug compound which is linked with the hyponatremia and protein metabolism. An elevated level of cholesterol contents observed in thiazide treated rats also revealed that the side effect of drug compound thiazide and also may be due to the stimulation of catecholamine which is stimulated therefore, the biochemical estimation such as protein and cholesterol in the testes after the thiazide treatment determined the effectiveness of diuretic drug compound would provide clinical evidences of their side effects.  相似文献   

虫媒登革病毒的研究——Ⅰ.Den 2病毒非结构蛋白NS1从感染细胞膜中的分离     

许琳  陈焕辉  刘乐和  郭辉玉 《中山大学学报论丛》1995,(2)

应用Con-A亲和层析方法从两株Den2病毒(New Guinea C株和从中国海南省分离的H-87株)感染的C6/36细胞膜中分离的糖蛋白,发现了一种感染细胞中特有的蛋白质。经SDS-PAGE证明该蛋白分子量约46000D,分析其为Den 2病毒的非结构蛋白NS1。并利用Western blot筛选出针对该蛋白4种单克隆抗体。  相似文献   

Ag2O/NaNbO3纳米复合材料的制备及其光催化性质     

李强  李世平  卢笑笑  马相东  韩浩  王康 《宜春学院学报》2020,(3):27-30

采用共沉淀法合成了Ag2O/NaNbO3复合光催化剂,并利用X射线衍射(XRD)和扫描电子显微镜(SEM)分别对其物相和形貌进行表征,用紫外可见漫反射光谱仪对其光学性质进行研究。在可见光下以罗丹明B(RhB)为目标降解物,检测了Ag2O/NaNbO3光催化剂的光催化性能。结果表明,与纯的Ag2O和纯的NaNbO3相比,Ag2O/NaNbO3复合光催化剂具有较高的光催化活性,其光催化活性的提升可能归结于p-n结的形成,使得光生电子空穴对的复合机率大大降低。  相似文献   

Experimental Study on Decolorization and Degradation of Reactive Brilliant Red X—3B in a White Rot Fungal Biofilm Reacor   总被引：1，自引：0，他引：1  

黄民生  黄荣  程永前  张国莹 《上海大学学报(英文版)》2001,5(3):260-264

Experimental results of an azo dye(reactive brilliant red X-3B,RBR X-3B) decolorization and degradation in a white rot fungal biofilm reactor were introduced and discussed.The fungal biofilm reactor is highly potential for dye decolorization and degradation with the highest decoloring rate of 95% within 96 hours reaction time at initial pH 4.5 under high nirogen level (HN) (24mmol/L ammonium tartrate)condition.Experimental conditions.such as nutrient nitrogen levels in reaction mixture and initial pH,significantly affected dye decolorization and degradation.Effluents from this bioilm reactor can be well treated to meet the discharging requirements by use of chemical flocculation.RBR X-3B was first absorbed onto fungal biomass and then degraded gradually,The SH-13 fungus monopolized the biofilm throughout the experiments,though the reactor was exposed to open air for 4 months.  相似文献   

植物中Ca2+调节的蛋白激酶的研究进展   总被引：1，自引：0，他引：1  

萧蓓蕾  冯建英  贾海慧  李冬梅 《宜春学院学报》2006,28(4):113-119

钙(Ca2 )是多种信号途径的第二信使,蛋白质磷酸化与脱磷酸化是信号传递的主要途径.蛋白激酶主要催化蛋白质的磷酸化作用,参与细胞信号识别与转导.Ca2 调节的蛋白激酶与细胞Ca2 信号进一步传递有密切的关系.  相似文献   

Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits   总被引：9，自引：0，他引：9  

Zhang M  Ma YF  Gan JX  Jiang GY  Xu SX  Tao XL  Hong A  Li JK 《Journal of Zhejiang University. Science. B》2005,6(7):637-643

The aim of this study was to explore the protective effect of basic fibroblast growth factor (bFGF) on brain injury following global ischemia reperfusion and its mechanisms. Brain injury following global ischemia was induced by four vessels occlusion and systemic hypotension. Twenty-four rabbits were randomized into three groups: group A, only dissection of vessels; group B, intravenous infusion of normal saline after reperfusion for 6 h; group C, 30 μg/kg bFGF injected intravenously at the onset of reperfusion, then infused with 10 μg/(kg·h) for 6 h. Serum neuron specific enolase (NSE), S-100B, tumor necrosis factor-α(TNF-α), interleukin-1 (IL-1), interleukin-8 (IL-8) were measured before ischemia, 30 min after ischemia, 0.5, 1, 3, 6 h after reperfusion. Brain water content was determined and cerebral histopathological damages were compared. NSE and S-100B were increased 1 h after reperfusion and reached their peaks 6 h after reperfusion, but were much higher in group B than those in group C 3, 6 h after reperfusion. In groups B and C, TNF-a was increased after ischemia and IL-1 and IL-8 were increased significantly 0.5 h after reperfusion, then reached their peaks 6 h, 3 h, 6 h after reperfusion respectively. TNF-a and IL-8 at the time points of 1 h and 3 h and IL-1 at 3 h and 6 h in group C were correspondingly lower than those in group B. These indices in group A were nearly unchanged. There were less severe cerebral histopathological damages in group C compared with group B, but no difference in brain water content. It could be concluded that bFGF alleviates brain injury following global ischemia and reperfusion by down-regulating expression of inflammatory factors and inhibiting their activities.  相似文献   

药物与蛋白质相互作用的研究新进展   总被引：2，自引：0，他引：2  

薛珺  饶美香  李蕾 《赣南师范学院学报》2002,(3):46-50

本文综述了药物与蛋白质相互作用的研究概况 ,内容涉及药物与蛋白质结合的意义 ,药物与蛋白质相互作用测定方法 ,结合部位、相互作用力确定等 ,涉及文献 2 5篇  相似文献   

"食物消化"实验改进与设计     

刘道琴 《遵义师范学院学报》2005,7(4):68-69

经过淀粉消化实验的改进、蛋白质和脂肪消化实验的新设计,使实验用材少、步骤简化,缩短实验时间,收到较好的实验效果.  相似文献